Over the past ten years or so, a great deal of emphasis has been placed on the use of bioluminescence technology in the detection of microorganisms.
The mechanism by which fireflies produce a flash of light was first analyzed and identified by William McElroy in 1947. McElroy found that central to the light emission process was a specific enzyme reaction catalyzing the consumption of adenosine triphosphate (ATP).
In microbes, ATP can only be detected when living cells are present. It has since been established that the amount of light emitted from this reaction is directly proportional to the amount of ATP present. A high reading of relative light units (RLUs) indicates that the sample contains a high number of micro-organisms, provided that the background ATP level is low.
Unlike traditional testing methods, results from a bioluminescent reaction can be obtained quickly. Light is produced within seconds and can be measured with a luminometer (ATP Meter).
The current palm-sized instruments bring together state-of-the-art photodiode technology with simple user-friendly design to produce an affordable hygiene-monitoring system. Used with ATP swabs, levels of contamination can be determined in just 15 seconds.
Key features of luminometers include, low cost, high sensitivity, compactness, simplicity in use, self- calibration with background check. Readings may be downloaded onto a PC or, as an optional extra, analysis software will provide spreadsheet-compatible data.
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